Accession Number : ADA614604


Title :   Methods to Analyze Bone Regenerative Response to Different rhBMP-2 Doses in Rabbit Craniofacial Defects


Descriptive Note : Journal article


Corporate Author : ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX


Personal Author(s) : Guda, Teja ; Darr, Aniq ; Silliman, David T ; Magno, Maria H ; Wenke, Joseph C ; Kohn, Joachim ; Baer, Pamela R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a614604.pdf


Report Date : 28 Feb 2014


Pagination or Media Count : 13


Abstract : Multiple assessment methods are available to evaluate the performance of engineered scaffolds in accepted bone healing animal models. Evaluation and comparison of these methods can aid in the planning of future animal studies, as well as, inform clinical assessments as the engineered scaffolds translate into clinical studies and applications. To evaluate multiple bone assessment techniques, bone regrowth potential of tyrosine-derived polycarbonate (TyrPC) scaffolds loaded with various dosages of recombinant human bone morphogenetic protein-2 (rhBMP-2) (0, 10, 25, and 50 mg) was assessed after 16 weeks in vivo in a rabbit calvarial model. Traditional X-ray radiography and micro-computed tomography (micro-CT) analyses were used to quantify the volume and density of regenerated bone. Histomorphometric analysis was performed as the traditional gold standard of evaluation. While these techniques are fairly standard in bone tissue engineering, we also investigated 64-slice CT, a tool more commonly used clinically, for comparison and to guide translational efforts. The 64-slice CT scans were carried out at 4 and 16 weeks to monitor temporal bone healing patterns. Study results indicated a clear dose-dependent response of increasing regenerated bone volume with rhBMP-2 loaded on the TyrPC scaffolds after 16 weeks of implantation. Significantly more bone formation was observed at the highest dose of rhBMP-2 (50 mg), which is 25 50% of the previously recommended dose (100 200 mg) for this defect. A significant difference was observed between the lowest and highest doses using radiographs ( p 0.001), micro-CT ( p 0.002), and CT ( p 0.001) and a high correlation was found between techniques (R2 values between 0.446 and 0.911).


Descriptors :   *BONES , *PROTEINS , *REGENERATION(PHYSIOLOGY) , COMPUTERIZED TOMOGRAPHY , DOSAGE , HISTOLOGY , POLYCARBONATES , RABBITS , RADIOGRAPHY , STATISTICAL ANALYSIS , SURGERY , TYROSINE


Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE