Accession Number : ADA614108


Title :   Axon-Sorting Multifunctional Nerve Guides: Accelerating Restoration of Nerve Function


Descriptive Note : Annual rept. 30 Sep 2013-29 Sep 2014


Corporate Author : GENEVA FOUNDATION TACOMA WA


Personal Author(s) : Alvarez, Luis


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a614108.pdf


Report Date : Oct 2014


Pagination or Media Count : 5


Abstract : Task 1: Engineer Laminin-collagen IV-entactin-perlecan (LCEP) binding domains as fusions to the growth and neurotrophic factors ( protein factors ). Generate LCEP composite fiber scaffolds incorporating protein factors exhibiting either constant surface density or longitudinal density gradients. Match fiber stiffness with that of autograft (G*1 MPa). Characterize the bioactivity of scaffold-bound protein factors using cell signaling assays (0-15 months); performance site: USMA. Task 2: Evaluate each protein factor (singly & in selected combinations) in the organotypic model system for preferential sensory or motor axon extension. Use confocal microscopy to track axon extension of labeled sensory or motor neurons from spinal cord slices (motor) or dorsal root ganglia (DRG) (sensory). 20 Thy1-YFP mice will be used for this task and subtasks. (12-24 mo.); sites: JHU & USMA Task 3: Evaluate top performing compositions from Task 2 in a rat femoral nerve repair model in which sensory and motor axons intermingle proximally and are segregated into discrete cutaneous and muscle branches distally. 80 Sprague-Dawley rats will be used for this task and subtasks. (24-36 mo.); work performed at JHU.


Descriptors :   *AXONS , *NERVES , *PROTEINS , *REGENERATION(PHYSIOLOGY) , ASSAYING , COLLAGEN , COMPOSITE MATERIALS , CONDUITS , FIBERS , INTERFACES , MICROSCOPY , PROSTHETICS , RATS , REPAIR , SPINAL CORD , STIFFNESS


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE