Accession Number : ADA613649


Title :   New Advanced Technologies in Stem Cell Therapy


Descriptive Note : Final rept. 1 Sep 2009-31 Aug 2014


Corporate Author : PITTSBURGH UNIV PA


Personal Author(s) : Huard, Johnny ; Fox, Ira ; Permutter, David


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a613649.pdf


Report Date : Nov 2014


Pagination or Media Count : 302


Abstract : We have isolated and characterized a population of skeletal muscle-derived stem cells (MDSCs) that display a greatly improved skeletal and cardiac muscle transplantation capacity when compared to skeletal muscle myoblasts. The MDSCs ability to withstand oxidative and inflammatory stresses appears to be the single most important factor for their improved transplantation capacity. Although the true origin of MDSCs remains unclear, their high degree of similarity with blood vessel-derived stem cells suggests their potential origin could be from the vascular wall. We have recently isolated two distinct populations of cells from the vasculature of human skeletal muscle known collectively as human skeletal muscle-derived cells (hMDCs). The two populations are myo-endothelial cells and pericytes and both can repair skeletal and cardiac muscles in a more effective manner than myoblasts, as is observed with murine MDSCs. In the current proposal we intend to evaluate and compare the regeneration capacity of these two hMDC populations after their implantation into the skeletal muscle of immunodeficient/dystrophic (SCID/mdx) mice. We will then investigate the influence that sex has on the regeneration and repair capacity of the hMDCs endowed with the greatest regeneration capacity (either myo-entothelial cells or pericytes). Finally we will investigate the influence that age plays on the regeneration capacity of the cells. Study Design: We will investigate the effects of cell survival, proliferation, resistance to stress, and neoangiogenesis on the regeneration capacity of the hMDCs implanted into the skeletal muscle of SCID/mdx mice Since we have observed that female murine MDSCs display an improved transplantation capacity in skeletal muscle when compared to male MDSCs, we will determine the influence that sex has on the hMDCs.


Descriptors :   *MUSCULOSKELETAL SYSTEM , *STEM CELLS , *SURGICAL TRANSPLANTATION , ANGIOGENESIS , APOPTOSIS , MICE


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE