Accession Number : ADA613491


Title :   Improving Joint Function Using Photochemical Hydrogels for Articular Surface Repair


Descriptive Note : Annual rept. 30 Sep 2013-29 Sep 2014


Corporate Author : MASSACHUSETTS GENERAL HOSPITAL BOSTON


Personal Author(s) : Randolph, Mark A ; Gill, Thomas J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a613491.pdf


Report Date : Oct 2014


Pagination or Media Count : 53


Abstract : The goal of our research is to introduce a novel means to regenerate the articular cartilage and restore normal function of the joint. A strategy that can generate durable hyaline articular cartilage, which will be predominantly type II collagen, and is capable of integrating with the surrounding cartilage matrix (without fissures) could improve the long-term outcome of joint surface repair. Key findings are isolation of MSCs and stimulation towards osteogenesis in vitro on an octacalcium phosphate scaffold showing cells populated the scaffold and calcium deposits demonstrated with von Kossa stains; 2) A degradable form of photochemically crosslinked PEG norbomene gel was formulated and growth factors (TGF beta) tethered to the polymer showing peri-cellular cartilage matrix around the encapsulated swine chondrocytes; 3) Formation of new cartilage matrix was demonstrated in vivo in mice using photochemically crosslinked gels and swine articular chondrocytes 4) Chondrocytes encapsulated in photochemically crosslinked hydrogels can survive the crosslinking and implantation process as shown in swine 5) Bone marrow derived mesenchymal stem cells from swine were encapsulated in alginate, stimulated with chondrogenic growth factors, and showed in vivo matrix productions


Descriptors :   *COLLAGEN , *JOINTS(ANATOMY) , CARTILAGE , CROSSLINKING(CHEMISTRY) , GELS , PHOTOCHEMICAL REACTIONS


Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE