Accession Number : ADA613412


Title :   Peripheral Nerve Repair and Prevention of Neuroma Formation


Descriptive Note : Annual progress rept. 1 Sep 2013-31 Aug 2014


Corporate Author : BAYLOR COLL OF MEDICINE HOUSTON TX


Personal Author(s) : Davis, Alan R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a613412.pdf


Report Date : Sep 2014


Pagination or Media Count : 46


Abstract : An overarching hypothesis of this application is that the ADRB3+ perineurial progenitor cells functionally contribute to both nerve and bone regeneration and during amputation these processes are aberrant leading to neuroma formation and HO. We proposed that neuroma formation could potentially be suppressed through selective modulation of neural inflammation. We have previously shown that inhibition of selective targets in this cascade can inhibit heterotopic ossification (HO). We established a model of neuroma in rats and found that delivery of cromolyn, suppressed the size of the neuromas. We proposed that cromolyn, which blocks mast cell degranulation, serotonin release, leads to the suppression of the ADRB3+ perineurial cells, and ultimately reduction or ablation of neuroma. The tissues are further being analyzed to confirm the molecular pathway has been altered as predicted. We are working on a system to measure the volume of the neuroma for comparison in these studies. Finally we have received several peripheral nerves from humans, and have been isolating the ADRB3+ cells, for characterization of selective markers as well as for culturing experiments in the rat. These are ongoing experiments. We have also received some human tissues from HO and have been analyzing the environment identified in these biopsies including the nerve structure which is present in the biopsy samples.


Descriptors :   *PERIPHERAL NERVOUS SYSTEM , ABLATION , ABNORMALITIES , AMPUTATION , BIOPSY , BONES , DELIVERY , ENVIRONMENTS , HUMANS , INHIBITION , MARKERS , MAST CELLS , MODELS , MODULATION , MOLECULES , NEOPLASMS , NERVES , NERVOUS SYSTEM , PREVENTION , REDUCTION , REGENERATION(PHYSIOLOGY) , RELEASE , REPAIR , SAMPLING , SEROTONIN , SUPPRESSION , TARGETS , TISSUES(BIOLOGY)


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE