Accession Number : ADA612268


Title :   Integrating and Amplifying Signal from Riboswitch Biosensors


Descriptive Note : Interim rept. 10 Jan 2012-1 Jan 2014


Corporate Author : UES INC DAYTON OH


Personal Author(s) : Goodson, Michael S ; Chushak, Yaroslav G ; Harbaugh, Svetlana V ; Kelley-Loughnane, Nancy


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a612268.pdf


Report Date : Aug 2014


Pagination or Media Count : 24


Abstract : Biosensors offer a built-in energy supply and inherent sensing machinery that when exploited correctly may surpass traditional sensors. However, biosensor systems have been hindered by a narrow range of ligand detection capabilities, a relatively low signal output, and their inability to integrate multiple signals. Integration of signals could increase the specificity of the sensor and enable detection of a combination of ligands that may indicate nefarious activities when detected together. Amplifying biosensor signal output will increase detector sensitivity and detection range. Riboswitches offer the potential to widen the diversity of ligands that may be detected, and advances in synthetic biology are illuminating myriad possibilities in signal processing using an orthogonal parts-based engineering approach. In this chapter we describe the design, building, and testing of a riboswitch-based Boolean logic AND gate in bacteria, where an output requires the activation of two riboswitches, and the biological circuitry required to amplify the output of the AND gate using natural extracellular bacterial communication signals to 'wire' cells together.


Descriptors :   *DETECTORS , BIOLOGICAL DETECTION , LIGANDS , SYNTHETIC BIOLOGY


Subject Categories : Genetic Engineering and Molecular Biology
      Miscellaneous Detection and Detectors


Distribution Statement : APPROVED FOR PUBLIC RELEASE