Accession Number : ADA610451


Title :   DNase 1 Retains Endodeoxyribonuclease Activity Following Gold Nanocluster Synthesis


Descriptive Note : Journal article


Corporate Author : ARMY RESEARCH LAB ABERDEEN PROVING GROUND MD


Personal Author(s) : West, Abby L ; Griep, Mark H ; Cole, Daniel P ; Karna, Shashi P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a610451.pdf


Report Date : 04 Jul 2014


Pagination or Media Count : 7


Abstract : Here we present the synthesis of the enzyme DNase 1 stabilized gold nanoclusters (DNase 1:AuNCs) with core size consisting of either 8 or 25 atoms. The DNase 1:Au8NCs exhibit blue fluorescence whereas the DNase 1:Au25NCs are red emitting. In addition to the intense fluorescence emission, the synthesized DNase 1:AuNC hybrid retains the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 microg/mL. The DNase 1:AuNCs could be conveniently employed as efficient and fast sensors to augment the current time-consuming DNA contamination analysis techniques.


Descriptors :   *FLUORESCENCE , *GOLD , *RIBONUCLEASE , ASSAYING , BIOMOLECULES , ENZYME CHEMISTRY , SYNTHESIS(CHEMISTRY)


Subject Categories : Biochemistry
      Physical Chemistry
      Miscellaneous Detection and Detectors


Distribution Statement : APPROVED FOR PUBLIC RELEASE