Accession Number : ADA609543


Title :   Brain Region and Cell Type Transcripts for Informative Diagnostics


Descriptive Note : Annual rept. 16 Jun 2009-9 Aug 2010


Corporate Author : INSTITUTE FOR SYSTEMS BIOLOGY SEATTLE WA


Personal Author(s) : Hood, Leroy E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a609543.pdf


Report Date : 01 Sep 2010


Pagination or Media Count : 42


Abstract : Our study will provide a set of candidate markers that can be used not only for the diagnosis of brain trauma, such as traumatic exposure to explosions, but also diseases such as brain cancer, including glioblastoma, and neural degenerative diseases, such as Alzheimer s disease. This year we used our EigenBrain approach to identify specific regions that are highly expressed in each cell type. In addition, we discovered a strong candidate set of brain-specific and cell-type specific transcripts. Moreover, we applied the region-based clustering method to the in situ hybridization of cell type specific genes. This revealed dramatic spatial consistency of neuron-specific genes, sufficient to recapitulate most anatomical brain regions from gene expression alone. We also analyzed brain RNAseq data to measure transcripts present in both human and mouse brains. This allowed us to detect the presence of many brain-specific genes (798 transcripts) that improved our candidate selection.


Descriptors :   *BRAIN , *CELLS(BIOLOGY) , *DIAGNOSIS(MEDICINE) , *TRANSCRIPTION(GENETICS) , FEATURE EXTRACTION , GENE EXPRESSION , GENES , IMAGES , RIBONUCLEIC ACIDS


Subject Categories : Genetic Engineering and Molecular Biology
      Biology
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE