Accession Number : ADA609244


Title :   The Importance of Neurogenic Inflammation in Blast-Induced Neurotrauma


Descriptive Note : Final rept. 1 Jan 2011-31 Dec 2013


Corporate Author : JOHNS HOPKINS UNIV LAUREL MD APPLIED PHYSICS LAB


Personal Author(s) : Wester, Brock ; Schaefer, Michele ; Boggs, Nathan ; Bradburne, Chris


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a609244.pdf


Report Date : May 2014


Pagination or Media Count : 72


Abstract : This study investigates the role of blood-borne immune cells as important mediators of damage and neuropathology resulting from blast-induced neurotrauma (BINT). We hypothesized that macrophages, along with their secreted cytokines and chemokines from the periphery, migrate via blood and infiltrate the CNS where they contribute to neuronal damage caused by activated microglia both in acute and chronic injury phases of BINT. We have used MRI and molecular techniques in mice with mild/moderate blast injury generated in a compressed helium-driven shock tube, as well as measurements of motor performance and behavioral assessment, observed temporally over 1 month. Groups of animals with mild/moderate BINT are imaged (MRI) and validated by immunocytochemistry, to visualize potential macrophage infiltration; blood-brain barrier (BBB) disturbance; reactive gliosis; or astrocyte activation. We show that a single exposure to mild/moderate blast induces both acute and chronic glial activation, levels of cytokines/chemokines, and motor impairment.


Descriptors :   *INFLAMMATION , *NEUROPHYSIOLOGY , *TRAUMA , ACTIVATION , ASTROCYTES , BARRIERS , BEHAVIOR , BLAST , BLOOD , BRAIN , CELLS(BIOLOGY) , CENTRAL NERVOUS SYSTEM , COMPRESSION , CYTOKINES , DAMAGE , EXPOSURE(GENERAL) , IMMUNITY , INFILTRATION(FLUIDS) , MACROPHAGES , MAGNETIC RESONANCE IMAGING , MEDICAL RESEARCH , MOLECULES , MOTOR DISORDERS , NERVE CELLS , NERVOUS SYSTEM , PATHOLOGY , PHASE , REACTIVITIES , SHOCK TUBES , VALIDATION , WOUNDS AND INJURIES


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE