Accession Number : ADA606215


Title :   Pegylated G-CSF Inhibits Blood Cell Depletion, Increases Platelets, Blocks Splenomegaly, and Improves Survival after Whole-Body Ionizing Irradiation but Not after Irradiation Combined with Burn


Descriptive Note : Journal article


Corporate Author : ARMED FORCES RADIOBIOLOGY RESEARCH INST BETHESDA MD


Personal Author(s) : Kiang, Juliann G ; Zhai, Min ; Liao, Pei-Jyun ; Bolduc, David L ; Elliott, Thomas B ; Gorbunov, Nikolai V


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a606215.pdf


Report Date : 05 Mar 2014


Pagination or Media Count : 12


Abstract : Exposure to ionizing radiation alone (radiation injury, RI) or combined with traumatic tissue injury (radiation combined injury, CI) is a crucial life-threatening factor in nuclear and radiological accidents. As demonstrated in animal models, CI results in greater mortality than RI. In our laboratory, we found that B6D2F1/J female mice exposed to 60Co-gamma-photon radiation followed by 15% total-body-surface-area skin burns experienced an increment of 18% highermortality over a 30-day observation period compared to irradiation alone; that was accompanied by severe cytopenia, thrombopenia, erythropenia, and anemia. At the 30th day after injury, neutrophils, lymphocytes, and platelets still remained very low in surviving RI and CI mice. In contrast, their RBC, hemoglobin, and hematocrit were similar to basal levels. Comparing CI and RI mice, only RI induced splenomegaly. Both RI and CI resulted in bone marrow cell depletion. It was observed that only the RI mice treated with pegylated G-CSF after RI resulted in 100% survival over the 30-day period, and pegylated G-CSF mitigated RI-induced body-weight loss and depletion of WBC and platelets. Peg-G-CSF treatment sustained RBC balance, hemoglobin levels, and hematocrits and inhibited splenomegaly after RI. The results suggest that pegylated G-CSF effectively sustained animal survival by mitigating radiation-induced cytopenia, thrombopenia, erythropenia, and anemia.


Descriptors :   *BLOOD CELLS , *BLOOD PLATELETS , *BURNS(INJURIES) , *SPLENOMEGALY , *WHOLE BODY IRRADIATION , BODY WEIGHT , IONIZING RADIATION , MICE


Subject Categories : Biochemistry
      Anatomy and Physiology
      Medicine and Medical Research
      Radiobiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE