Accession Number : ADA603817


Title :   The Polysaccharide Capsule of Campylobacter jejuni 81-176 Modulates the Host Immune Response


Descriptive Note : Journal article


Corporate Author : TEXAS UNIV AT AUSTIN


Personal Author(s) : Maue, Alexander C ; Mohawk, Krystle L ; Giles, David K ; Poly, Frederic ; Ewing, Cheryl P ; Jiao, Yuening ; Lee, Ginyoung ; Ma, Zuchao ; Monteiro, Mario A ; Hill, Christina L


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a603817.pdf


Report Date : 17 Dec 2012


Pagination or Media Count : 42


Abstract : Campylobacter jejuni is a major cause of bacterial diarrheal disease worldwide. The organism is characterized by a diversity of polysaccharide structures, including a polysaccharide capsule. Most C. jejuni capsules are known to be decorated non-stoichiometrically with methyl phosphoramidate (MeOPN). The capsule of C. jejuni 81-176 has been shown to be required for serum resistance, but here we show that an encapsulated mutant lacking the MeOPN modification, mpnC, was equally sensitive to serum killing as the non29 encapsulated mutant. A non-encapsulated mutant, kpsM, exhibited significantly reduced colonization compared to wildtype 81-176 in a mouse intestinal colonization model, and the mpnC mutant showed an intermediate level of colonization. Both mutants were associated with higher levels of IL-17 expression from lamina propria CD4+ cells compared to cells from animals infected with 81-176. In addition, reduced levels of TLR-4 and -2 activation were observed following in vitro stimulation of human reporter cell lines with kpsM and mpnC compared to wildtype 81-176. The data suggest that the capsule polysaccharide of C. jejuni, and the MeOPN modification, modulate the host immune response.


Descriptors :   *JEJUNUM , *POLYSACCHARIDES , BACTERIA , CELLS(BIOLOGY) , DIARRHEA , ENCAPSULATION , IMMUNITY , IN VITRO ANALYSIS , METHYL RADICALS , RESPONSE(BIOLOGY) , STIMULATION(PHYSIOLOGY)


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE