Accession Number : ADA601521


Title :   The Insect SNMP Gene Family


Descriptive Note : Journal article


Corporate Author : SOUTH CAROLINA UNIV COLUMBIA DEPT OF BIOLOGICAL SCIENCES


Personal Author(s) : Vogt, Richard G ; Miller, Natalie E ; Litvack, Rachel ; Fandino, Richard A ; Sparks, Jackson ; Staples, Jon ; Friedman, Robert ; Dickens, Joseph C


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a601521.pdf


Report Date : Jan 2009


Pagination or Media Count : 11


Abstract : SNMPs are membrane proteins observed to associate with chemosensory neurons in insects; in Drosophila melanogaster, SNMP1 has been shown to be essential for the detection of the pheromone cisvaccenyl acetate (CVA). SNMPs are one of three insect gene clades related to the human fatty acid transporter CD36. We previously characterized the CD36 gene family in 4 insect Orders that effectively cover the Holometabola, or some 80% of known insect species and the 300 million years of evolution since this lineage emerged: Lepidoptera (e.g. Bombyx mori, Antheraea polyphemus, Manduca sexta, Heliothis virescens, Helicoverpa assulta, Helicoverpa armigera, Mamestra brassicae); Diptera (D. melanogaster Drosophila pseudoobscura, Aedes aegypti, Anopheles gambiae, Culex pipiens quinquefasciatus); Hymenoptera (Apis mellifera); and Coleoptera (Tribolium castaneum). This previous study suggested a complex topography within the SNMP clade including a strongly supported SNMP1 sub-clade plus additional SNMP genes. To further resolve the SNMP clade here, we used cDNA sequences of SNMP1 and SNMP2 from various Lepidoptera species, D. melanogaster and Ae. aegypti, as well as BAC derived genomic sequences from Ae. aegypti as models for proposing corrected sequences of orthologues in the D. pseudoobscura and An. gambiae genomes, and for identifying orthologues in the B. mori and C. pipiens q. genomes. We then used these sequences to analyze the SNMP clade of the insect CD36 gene family supporting the existence of two well supported sub-clades, SNMP1 and SNMP2, throughout the dipteran and lepidopteran lineages, and plausibly throughout the Holometabola and across a broad evolutionary time scale. We present indirect evidence based on evolutionary selection (dN/dS) that the dipteran SNMPs are expressed as functional proteins.


Descriptors :   *GENES , *MEMBRANES , *PROTEINS , CULICIDAE , FATTY ACIDS , NERVE CELLS , PHEROMONES


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE