Accession Number : ADA599371


Title :   Whole Adult Organism Transcriptional Profiling of Acute Metal Exposures in Male Zebrafish


Descriptive Note : Research 1 Jan 2007-3 Jan 2014


Corporate Author : ARMY CENTER FOR ENVIRONMENTAL HEALTH RESEARCH FORT DETRICK MD


Personal Author(s) : Hussainzada, Naissan ; Lewis, John A ; Baer, Christine E ; Ippolito, Danielle L ; Jackson, David A ; Stallings, Jonathan D


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a599371.pdf


Report Date : 10 Mar 2014


Pagination or Media Count : 16


Abstract : A convergence of technological breakthroughs in the past decade has facilitated the development of rapid screening tools for biomarkers of toxicant exposure and effect. Platforms using the whole adult organism to evaluate the genome-wide response to toxicants are especially attractive. Recent work demonstrates the feasibility of this approach in vertebrates using the experimentally robust zebrafish model. In the present study, we evaluated gene expression changes in whole adult male zebrafish following an acute 24 hr high dose exposure to three metals with known human health risks. Male adult zebrafish were exposed to nickel chloride, cobalt chloride or sodium dichromate concentrations corresponding to their respective 96 hr LC20, LC40 and LC60. Histopathology was performed on a subset of metal-exposed zebrafish to phenotypically anchor transcriptional changes associated with each metal.


Descriptors :   *CHLORIDES , *CHROMATES , *FISHES , *METALS , *NICKEL , *TOXICITY , AQUATIC ORGANISMS , COBALT COMPOUNDS , GENES , HEALTH , HISTOPATHOLOGY , SODIUM , TOOLS , TRACER STUDIES , VERTEBRATES


Subject Categories : Biology
      Toxicology
      Inorganic Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE