Accession Number : ADA599245


Title :   Hetero-oligonucleotide Nanoscale Tiles Capable of Two-Dimensional Lattice Formation as Testbeds for a Rapid, Affordable Purification Methodology


Descriptive Note : Journal article


Corporate Author : SAINT JOHN'S UNIV JAMAICA NY


Personal Author(s) : Lukeman, Philip S


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a599245.pdf


Report Date : Jan 2013


Pagination or Media Count : 6


Abstract : New nanoscale hetero-oligonucleotide tiles are assembled from DNA, RNA and morpholino oligos and purified using size exclusion filtration. Homo-oligonucleotide tiles assembled from RP-cartridge processed DNA oligos are purified by nondenaturing gel electro- phoresis. These tiles' purity and homogeneity are demonstrated by gel electrophoresis and their incorporation into two-dimensional arrays visualized by AFM. This purification methodology increases throughput and decreases costs for researchers who wish to screen multiple tiles for utilization in structural or analytical studies.


Descriptors :   *ARRAYS , *DEOXYRIBONUCLEIC ACIDS , *NANOTECHNOLOGY , *NUCLEOTIDES , *PURIFICATION , ELECTROPHORESIS , FILTRATION , RIBONUCLEIC ACIDS , TEST BEDS , TILES , TWO DIMENSIONAL


Subject Categories : Biochemistry
      Industrial Chemistry and Chemical Processing
      Organic Chemistry
      Electrical and Electronic Equipment


Distribution Statement : APPROVED FOR PUBLIC RELEASE