Accession Number : ADA598723


Title :   Isolation and Characterization of Prostate Cancer Stem Cells


Descriptive Note : Annual summary 1 Aug 2008-31 Jul 2013


Corporate Author : CALIFORNIA UNIV LOS ANGELES


Personal Author(s) : Garraway, Isla


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a598723.pdf


Report Date : Oct 2013


Pagination or Media Count : 138


Abstract : The aims of this proposal are based on the observations from our initial studies with human prostate cancer surgical specimens. We discovered that prostate stem/early progenitor cells recovered from tumor specimens lack the TMPRSS-ERG translocation found in the original tumor, when examined by fluorescence in situ hybridization (FISH). Our findings suggest either ETS rearrangements are not present at the stem/progenitor cell level, or that genetically deranged prostate stem/progenitor cells are particularly vulnerable to apoptosis or senescence in vitro, resulting in selective advantage of benign cells. We have evaluated the growth requirements that enable survival and expansion of prostate stem/progenitor cells from benign and malignant human tissue specimens. As such, we have generated an extensive biorepository of human prostate tissue and/or primary cell lines from more than 500 prostate cancer cases that may serve as provide valuable biological tools for understanding the mechanisms of tumorigenesis and identifying new therapeutic targets in future studies. Factors that have affected the ability to isolate and expand primary tumors include low tumor grade/volume present in surgical samples, increased sensitivity of tumor cells to apoptosis after tissue dissociation, semi-competent immune systems in many SCID mouse strains that inhibit tumor take, and a microenvironment that lacks critical growth factors and cellular interactions.


Descriptors :   *PROSTATE CANCER , *STEM CELLS , CELLS(BIOLOGY) , GROWTH(PHYSIOLOGY) , ISOLATION , THERAPY , TISSUES(BIOLOGY)


Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE