Accession Number : ADA597879


Title :   Novel in Vitro Modification of Bone for an Allograft with Improved Toughness Osteoconductivity


Descriptive Note : Annual rept. 30 Sep 2012-29 Sep 2013


Corporate Author : HENRY FORD HEALTH SYSTEM DETROIT MI


Personal Author(s) : Yeni, Yener N


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a597879.pdf


Report Date : Oct 2013


Pagination or Media Count : 20


Abstract : The purpose of the project is to investigate a potential application of ALT-711 (4,5-Dimethyl-3-(2-oxo-2-phenylethyl)-thiazolium chloride) in improving both mechanical and biological quality of femoral cortical bone for the purpose of providing better allograft materials via chemically breaking down AGEs (Advanced Glycation Endproducts) in the bone matrix. AGEs are naturally accumulated with age in connective tissues and have adverse effects on the biological and mechanical functions. Thus allografts sourced from bones with a high level of AGEs are likely to have a higher risk of nonunion and premature failure in the hosts. The current scope, as a proof of concept study, is to measure the effectiveness of ALT-711 on both cellular and mechanical characteristics of bones with and without prior glycation treatment (an artificial method to increase AGEs). We will measure effects of ALT-711 on osteogenic expression of stem cells on the bone substrates and also measure fracture toughness, a key measure of strong bone. We have demonstrated thus far ALT-711 has weak but measurable positive effects on the osteogenic expression of stem cells on bones without ribose treatment. We also have demonstrated ALT-711 is able to reverse the fluorescence level (assumed to be correlated to the amount of AGEs) previously increased by glycation. As such, we are encouraged by the preliminary data and now focusing our efforts in completing rest of the tasks assigned to the project. We expect to complete the work in the next period.


Descriptors :   *OSTEOGENESIS , *OSTEOPOROSIS , BONES , CEREBRAL CORTEX , FAILURE , RIBOSE , RISK , SURGICAL TRANSPLANTATION , TOUGHNESS


Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE