Accession Number : ADA595708


Title :   In Vivo Multimodal Imaging of NF-kappaB (pB) Spatial and Temporal Activation Following Light Injury in the Mouse Retina


Descriptive Note : Interim rept. Jan 2010-Jan 2012


Corporate Author : AIR FORCE RESEARCH LAB FORT SAM HOUSTON TX HUMAN PERFORMANCE WING (711TH) HUMAN EFFECTIVENESS DIR/ DIRECTED ENERGY BIOEFFECTS DIVISION


Personal Author(s) : Pocock, Ginger M ; Lavey, Brent J ; Boretsky, Adam ; Gupta, Praveena ; Vargas, Gracie ; Zhang, Wenbo ; Motamedi, Massoud ; Wang, Heuy-Ching H


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a595708.pdf


Report Date : 15 Jan 2014


Pagination or Media Count : 46


Abstract : BALB/c and C57BL/6 wild type mice and (cis-NF- B-EGFP) transgenic mouse model were used to perform in vivo experiments of laserinduced thermal damage to the retina. A Heidelberg Spectralis HRA confocal scanning laser ophthalmoscope with a spectral domain optical coherence tomographer was used to obtain fundus and cross-sectional images of laser induced injury in the retina. Sub-threshold, threshold, and supra-threshold lesions were imaged up to 10 days post exposure using optical coherence tomography (OCT), infrared reflectance, red-free reflectance, fluorescence angiography, and autofluorescence imaging modalities. The spatial and temporal activation of Nuclear factor-kappa B (NF- B) (p65) was monitored in the retina of the cis-NF- B-EGFP mouse. In addition, EGFP basal expression in brain and retinal tissue from the cis-NF- B-EGFP was characterized using two-photon imaging. Injured and control eyes were enucleated at discrete time points following laser exposure for cryosectioning and confocal microscopy to determine localization of NF- B dependent enhanced green fluorescent protein (EGFP) reporter gene expression within the retina. Furthermore, immunohistochemistry for GFAP, IBA-1, NeuN, CD65, and glutamine synthetase were used to examine the retinal structures and cell population expressing upregulated activation of p65 as a result of laser induced injury.


Descriptors :   *LASER INDUCED FLUORESCENCE , *MICE , *RETINA , *WOUNDS AND INJURIES , CELLS(BIOLOGY) , COHERENCE , CROSS SECTIONS , DAMAGE , EYE , GENES , HISTOCHEMISTRY , IMAGES , IMMUNOCHEMISTRY , IN VIVO ANALYSIS , INFRARED RADIATION , LASERS , OPHTHALMOLOGY , OPTICAL PROPERTIES , OPTICAL SCANNING , PHOTONS , REFLECTANCE , SCANNING ELECTRON MICROSCOPES , SPATIAL DISTRIBUTION , TISSUES(BIOLOGY) , TOMOGRAPHY


Subject Categories : Anatomy and Physiology
      Radiation and Nuclear Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE