Accession Number : ADA594898


Title :   CINRG: Systems Biology of Glucocorticoids in Muscle Disease


Descriptive Note : Final rept. 21 Sep 2009-20 Sep 2013


Corporate Author : CHILDREN'S RESEARCH INST WASHINGTON DC CENTER FOR GENETIC MEDICINE


Personal Author(s) : Wang, Zuyi


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a594898.pdf


Report Date : Oct 2013


Pagination or Media Count : 18


Abstract : We developed two computational network reconstruction methods, linear state space model and dynamic Bayesian network, to infer transcriptional networks using the rat acute transcriptional time series of bolus administration of glucocorticoids. This time series was re-profiled using Illumina gene expression BeadChip for a much broader coverage. We have successfully used staged injection models to induce asynchronous regenerations in normal mouse muscles. Using laser capture microscopy and gene expression microarray profiling, we extensively analyzed muscle tissues dissected from the injection sites and in between regions in the 4 day and 10 day reinjury series. The results showed inappropriate crosstalk in the muscles from in between areas due to neighboring asynchronous regenerations in both injection series. We showed that daily administration of Prednisolone suppressed inappropriate crosstalk in the muscle regions between asynchronously remodeling areas. We have been awarded several new grants focusing on studying glucocorticoid mechanism in treating DMD and asthma. Two doctoral students graduated or are graduating under partial support from this grant.


Descriptors :   *BAYES THEOREM , *DISEASES , *GENE EXPRESSION , *GENES , *MUSCLES , CROSSTALK , DAILY OCCURRENCE , INJECTION , LASERS , MATHEMATICAL MODELS , MICROSCOPY , STUDENTS , SUPPRESSION , TISSUES(BIOLOGY)


Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research
      Numerical Mathematics


Distribution Statement : APPROVED FOR PUBLIC RELEASE