Accession Number : ADA593300


Title :   The Role of a Novel Myosin Isoform in Prostate Cancer Metastasis


Descriptive Note : Annual rept. 30 Sep 2012-29 Sep 2013


Corporate Author : STATE UNIV OF NEW YORK COLL AT BUFFALO


Personal Author(s) : Hofmann, Wilma A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a593300.pdf


Report Date : Oct 2013


Pagination or Media Count : 31


Abstract : The objective of the project for the reporting period was to generate a number prostate cancer cell lines that that are either myosin IC isoform A deficient or that constitutively express GFP-myosin IC isoform A. We used shRNA to generate isoform Anegative PC-3 cells and we generated a stable LNCaP cell line that expresses constitutively myosin IC isoform A-GFP. We are now in the process of analyzing the effect of these expression changes on secretion in these cell lines to determine the consequences of isoform A expression changes for the metastatic ability of prostate cancer cells. Using serial cloning of the 5 prime UTR of the human myosin IC gene we have identified a region that is involved in regulatory expression of myosin IC isoform A. We are now in the process of using ChIP assays to identifying the transcriptional elements that bind to this region and induce expression of myosin IC isoform A in prostate cancer cells.


Descriptors :   *PROSTATE CANCER , GENES , METASTASIS , MYOSIN


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE