Accession Number : ADA593283


Title :   FGFR4 Downregulation of Cell Adhesion in Prostate Cancer


Descriptive Note : Final rept.


Corporate Author : CALIFORNIA UNIV SAN DIEGO LA JOLLA


Personal Author(s) : Donoghue, Daniel J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a593283.pdf


Report Date : Sep 2008


Pagination or Media Count : 13


Abstract : We have been successful in creating the necessary constructs for generating prostate cancer cells inducible for FGFR4. Despite our best efforts, however, we have not been able to successfully incorporate the pVgRXR regulatory plasmid into prostate cancer cells. We proceeded by transiently transfecting PC3 prostate cancer cells to look at effects on downstream signaling components and found no significant differences in MAPK activity, NCAM expression or STAT1 and STAT5 localization when comparing the various FGFR4 constructs, including FL-FGFR4 and PTD-FGFR4. We have also made a very exciting discovery recently that may be important for understanding the role of FGFR4 in prostate cancer progression. Using a yeast-two-hybrid assay, we found that FGFR4 interacts with IKKbeta and leads to its tyrosine phosphorylation. We are currently investigating if there are any differences in binding tyrosine phosphorylation with the G388R polymorphism. Though not specifically relating to cell adhesion, understanding the role of FGFR4 in altering IKKbeta activity may be important for our understanding of prostate cancer progression.


Descriptors :   *ADHESION , *CELLS , *PROSTATE CANCER , POLYMORPHISM , TYROSINE


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research
      Physical Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE