Accession Number : ADA592835


Title :   Modulation of TIP60 by Human Papilloma Virus in Breast Cancer


Descriptive Note : Annual summary 15 Aug 2011-24 Jan 2013


Corporate Author : VIRGINIA UNIV CHARLOTTESVILLE


Personal Author(s) : Gupta, Ashish


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a592835.pdf


Report Date : Apr 2013


Pagination or Media Count : 65


Abstract : We examined the role of HPVE6 on the modulation of Tip60 in Breast epithelial cells. HPVE6 oncoprotein can destabilize the Histone acetyl transferase TIP60 in breast epithelial cells. Using the inhibitors of proteasomal pathway we found that the destabilization of Tip60 by E6 is through its protein degradation. Our Ubiquitination assays result suggested that this degradation of TIP60 might not involve the polyubiquitination dependent post translational modification. We hypothesize that E6 might facilitate the interaction of TIP60 with its E3 ligase and thus leads to its depletion. We aim to find the E3 ligase involved in E6 mediated TIP60 degradation. We intend to examine the role of TIP60 degradation by HPV virus and its significance in malignant transformation of the breast epithelial cells.


Descriptors :   *BREAST CANCER , *CELLS(BIOLOGY) , ACETYL CHLORIDE , DEGRADATION , DEPLETION , EPITHELIUM , HISTONES , INHIBITORS , INTERACTIONS , MAMMARY GLANDS , MEDICAL RESEARCH , MODIFICATION , MODULATION , PROTEINS , TRANSFERASES , TRANSFORMATIONS , VIRUSES


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE