Accession Number : ADA583966


Title :   Morphological and Functional Differentiation in BE (2)-M17 Neuroblastoma Cells by Treatment with Trans-Retinoic Acid


Descriptive Note : Journal article


Corporate Author : ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD


Personal Author(s) : Andres, Devon ; Keyser, Brian M ; Petrali, John ; Benton, Betty ; Hubbard, Kyle S ; McNutt, Patrick M ; Ray, Radharaman


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a583966.pdf


Report Date : 18 Apr 2013


Pagination or Media Count : 13


Abstract : Background: Immortalized neuronal cell lines can be induced to differentiate into more mature neurons by adding specific compounds or growth factors to the culture medium. This property makes neuronal cell lines attractive as in vitro cell models to study neuronal functions and neurotoxicity. The clonal human neuroblastoma BE(2)-M17 cell line is known to differentiate into a more prominent neuronal cell type by treatment with trans-retinoic acid. However, there is a lack of information on the morphological and functional aspects of these differentiated cells. Results: We studied the effects of trans-retinoic acid treatment on (a) some differentiation marker proteins, (b) types of voltage-gated calcium (Ca2+) channels and (c) Ca2+-dependent neurotransmitter ([3H] glycine) release in cultured BE(2)-M17 cells. Cells treated with 10 M trans-retinoic acid (RA) for 72 hrs exhibited marked changes in morphology to include neurite extensions; presence of P/Q, N and T-type voltage-gated Ca2+ channels; and expression of neuron specific enolase (NSE), synaptosomal-associated protein 25 (SNAP-25), nicotinic acetylcholine receptor 7 (nAChR- 7) and other neuronal markers. Moreover, retinoic acid treated cells had a significant increase in evoked Ca2+-dependent neurotransmitter release capacity. In toxicity studies of the toxic gas, phosgene (CG), that differentiation of M17 cells with RA was required to see the changes in intracellular free Ca2+ concentrations following exposure to CG. Conclusion: Taken together, retinoic acid treated cells had improved morphological features as well as neuronal characteristics and functions; thus, these retinoic acid differentiated BE(2)-M17 cells may serve as a better neuronal model to study neurobiology and/or neurotoxicity.


Descriptors :   *NERVE CELLS , *RETINOIC ACIDS , CALCIUM , CULTURES(BIOLOGY) , DEPOLARIZATION , MICROSCOPY , MORPHOLOGY , NEUROTRANSMITTERS , PHOSGENE , PROTEINS , SYNAPSE , TOXICITY


Subject Categories : Anatomy and Physiology
      Organic Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE