Accession Number : ADA583418


Title :   Differentiation of Neonatal Human-Induced Pluripotent Stem Cells to Prostate Epithelial Cells: A Model to Study Prostate Cancer Development


Descriptive Note : Annual rept. 7 May 2012-6 May 2013


Corporate Author : WISCONSIN UNIV MADISON


Personal Author(s) : Setaluri, Vijayasaradhi


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a583418.pdf


Report Date : Jun 2013


Pagination or Media Count : 20


Abstract : In this project we set out to establish conditions for differentiation of human neonatal foreskin skin fibroblast-derived iPSCs into prostate epithelium-like cells and identify differences in gene expression between prostate epithelial cells derived from iPSCs of Caucasian (white) and African-American (black) foreskin fibroblasts. We identified and optimized culture conditions that promote prostate epithelial cell-like differentiation of humaniPS clone, IMAR90-4. Our data show that a feeder layer of urogential mesenchymal(UGSM) cells from neonatal mouse of either gender in combination with neonatal human dermal fibroblasts induced a striking morphological changes that resembles epithelila differentiation with formation of lumen-like structures. We show requirement of components of the extracellular matrix that promote epithelial-type differentaition. Immunofluorescence and biochemivcal analyses showed expression of androgen receptor and markers of epithelial differentiation. Analyses of pluripotency marker expression by RT-PCR showed that while human dermal fibroblasts have higher constitutive expression of Nanog, Oct4 and Sox2 compared to UGSM and IMP90 cells. Preliminary studies also showed that black black fibrobalst population has higher constitutive expression of pluripotency markers than cells from white individuals.


Descriptors :   *PROSTATE CANCER , *STEM CELLS , AFRICAN AMERICANS , BLOOD CELLS , CELLS(BIOLOGY) , EPITHELIUM , FIBROBLASTS , GENOME , HEMATOPOIETIC CELLS , HUMANS , MARKERS , MODELS , OPTIMIZATION , PROSTATE GLAND , RISK


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE