Accession Number : ADA581893


Title :   Unbiased Combinatorial Genomic Approaches to Identify Alternative Therapeutic Targets within the TSC Signaling Network


Descriptive Note : Annual rept. 1 Jun 2012-31 May 2013


Corporate Author : HARVARD UNIV BOSTON MA


Personal Author(s) : Perrimon, Norbert ; Manning, Brendan


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a581893.pdf


Report Date : Jun 2013


Pagination or Media Count : 34


Abstract : The long-term goal of this project is geared toward further defining the regulatory mechanisms impinging on the TSC-Rheb circuit and revealing therapeutic strategies to target this signaling network in genetic tumor syndromes and cancer. Specifically, we aim to establish a robust synthetic lethal screening method applied to the study of the TSC network, validate synthetic lethal pairs in an in vivo intestinal stem cell system, and determine whether synthetic lethal combinations found in Drosophila are relevant to mammalian networks. In the first year of funding we have made significant progress at establishing the tools needed for the synthetic screens both in tissue culture and in vivo.


Descriptors :   *COMBINATORIAL ANALYSIS , *DROSOPHILA , *GENOME , *IN VIVO ANALYSIS , *RIBONUCLEIC ACIDS , *STEM CELLS , LETHALITY , MAMMALS , NEOPLASMS , NETWORKS , SIGNS AND SYMPTOMS , SYNTHESIS(CHEMISTRY) , THERAPY , TISSUE CULTURE


Subject Categories : Genetic Engineering and Molecular Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE