Accession Number : ADA580337

Title :   Analysis of the Effects of a gerP Mutation on the Germination of Spores of Bacillus subtilis

Descriptive Note : Journal article


Personal Author(s) : Butzin, Xuan Y ; Troiano, Anthony J ; Coleman, William H ; Griffiths, Keren K ; Doona, Christopher J ; Feeherry, Florence E ; Wang, Guiwen ; Li, Yong-Qing ; Setlow, Peter

Full Text :

Report Date : Nov 2012

Pagination or Media Count : 14

Abstract : As previously reported, gerP Bacillus subtilis spores were defective in nutrient germination triggered via various germinant receptors (GRs), and the defect was eliminated by severe spore coat defects. The gerP spores GR-dependent germination had a longer lag time between addition of germinants and initiation of rapid release of spores dipicolinic acid (DPA), but times for release of 90% of DPA from individual spores were identical for wild-type and gerP spores. The gerP spores were also defective in GR-independent germination by DPA with its associated Ca2+ divalent cation (CaDPA) but germinated better than wild-type spores with the GR-independent germinant dodecylamine. The gerP spores exhibited no increased sensitivity to hypochlorite, suggesting that these spores have no significant coat defect. Overexpression of GRs in gerP spores did lead to faster germination via the overexpressed GR, but this was still slower than germination of comparable gerP spores. Unlike wild-type spores, for which maximal nutrient germinant concentrations were between 500 microM and 2mM for L-alanine and /+10mM for L-valine, rates of gerP spore germination increased up to between 200 mM and 1M L-alanine and 100mM L-valine, and at 1M L-alanine, the rates of germination of wild-type and gerP spores with or without all alanine racemases were almost identical. A high pressure of 150 MPa that triggers spore germination by activating GRs also triggered germination of wild-type and gerP spores identically. All these results support the suggestion that GerP proteins facilitate access of nutrient germinants to their cognate GRs in spores inner membrane.


Subject Categories : Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE