Accession Number : ADA580295


Title :   Genetics of Persister Formation in Pseudomonas aeruginosa


Descriptive Note : Final rept. 1 Jun 2009-31 May 2012


Corporate Author : NORTHEASTERN UNIV BOSTON MA OFFICE OF SPONSORED PROJECTS


Personal Author(s) : Lewis, Kim


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a580295.pdf


Report Date : 14 Dec 2012


Pagination or Media Count : 14


Abstract : Our original proposal was aimed at understanding the mechanism of how Pseudomonas aerugionsa forms drug tolerant persister cells. This is of critical importance because persisters can lead to antimicrobial therapy failure. Specific Aim 1: Transcriptomic analysis of stationary phase persisters. We successfully developed a method to isolate P. aerugionsa persisters and obtained total RNA from these cells. Attempts to obtain a transcriptome with this RNA failed due to the limitations of our samples. However, the methods developed from this work allowed us to obtain the first persister proteome. This achievement is an important technical and scientific advance. The results have given us candidate persister genes that we are now working to validate. Additionally, these results will provide a guide for other groups working on proteomics approaches where material is limited. Specific Aim 2: Genomic Identification of High Persister (hip) Mutants. We completed this aim and obtained genome sequences of hip mutants from clinical isolates.


Descriptors :   *CELLS(BIOLOGY) , *GENETICS , *PSEUDOMONAS AERUGINOSA , ANTIBIOTICS , DRUG TOLERANCE , RIBONUCLEIC ACIDS


Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE