Accession Number : ADA579442


Title :   Phytoremediation of Atmospheric Methane


Descriptive Note : Final rept. 1 Apr 2010 - 31 Mar 2012


Corporate Author : WASHINGTON UNIV SEATTLE


Personal Author(s) : Strand, Stuart ; Bruce, Neil ; Rylott, Liz ; Zhang, Long


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a579442.pdf


Report Date : 15 Apr 2013


Pagination or Media Count : 40


Abstract : We have transformed a plant, Arabidopsis thaliana, with the bacterial genes for the three subunit proteins of the soluble methane monooxygenase and with two associated genes, the mmoG and the mmoC. The presence of the genes and the transcripts in selected lines of the transformed plants was confirmed at the genomic, transcript and protein levels. The transformed plants removed more methane compared to the wild-type, but only slightly, about 5% in 7 days . The production of 1-naphthol from naphthalene was 2-3 times greater in transformed plant lines compared to untransformed wild types. These results suggest that the sMMO was properly assembled and partially functional, however, additional controls are needed to confirm. We discuss the results and suggest follow-on experiments.


Descriptors :   *BACTERIA , *METHANE , *PLANTS(BOTANY) , GENES , GREENHOUSE EFFECT , PROTEINS , TRANSFORMATIONS , WILDLIFE


Subject Categories : Microbiology
      Organic Chemistry


Distribution Statement : APPROVED FOR PUBLIC RELEASE