Accession Number : ADA576680


Title :   Autonomous Bacterial Localization and Gene Expression Based on Nearby Cell Receptor Density


Descriptive Note : Journal article


Corporate Author : ARMY RESEARCH LAB ADELPHI MD SENSORS AND ELECTRON DEVICES DIRECTORATE


Personal Author(s) : Wu, Hsuan-Chen ; Tsao, Chen-Yu ; Quan, David N ; Cheng, Yi ; Servinsky, Matthew D ; Carter, Karen K ; Jee, Kathleen J ; Terrell, Jessica L ; Zargar, Amin ; Rubloff, Gary W


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a576680.pdf


Report Date : 22 Jan 2013


Pagination or Media Count : 9


Abstract : Escherichia coli were genetically modified to enable programmed motility, sensing, and actuation based on the density of features on nearby surfaces. Then, based on calculated feature density, these cells expressed marker proteins to indicate phenotypic response. Specifically, site-specific synthesis of bacterial quorum sensing autoinducer-2 (AI-2) is used to initiate and recruit motile cells. In our model system, we rewired E. coli's AI-2 signaling pathway to direct bacteria to a squamous cancer cell line of head and neck (SCCHN), where they initiate synthesis of a reporter (drug surrogate) based on a threshold density of epidermal growth factor receptor (EGFR). This represents a new type of controller for targeted drug delivery as actuation (synthesis and delivery) depends on a receptor density marking the diseased cell. The ability to survey local surfaces and initiate gene expression based on feature density represents a new area-based switch in synthetic biology that will find use beyond the proposed cancer model here.


Descriptors :   *GENE EXPRESSION , *RECEPTOR SITES(PHYSIOLOGY) , CANCER , ESCHERICHIA COLI , SYNTHETIC BIOLOGY


Subject Categories : Genetic Engineering and Molecular Biology
      Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE