Accession Number : ADA570695


Title :   Stabilization of RNA through Absorption by Functionalized Mesoporous Silicate Nanospheres


Descriptive Note : Journal article


Corporate Author : NAVAL RESEARCH LAB WASHINGTON DC CENTER FOR BIOMOLECULAR SCIENCE AND ENGINEERING


Personal Author(s) : Johnson, Brandy J ; Melde, Brian J ; Dinderman, Michael A ; Lin, Baochuan


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a570695.pdf


Report Date : 30 Nov 2012


Pagination or Media Count : 8


Abstract : The potential for encapsulating RNA within tunable, semi-permeable structures for storage and transportation purposes offers an interesting approach to the reduction of stringent storage requirements that often hamper the field application of genetic analysis methods. In this study, we assessed the potential for application of functionalized, porous silicate sorbents in maintaining nucleic acid integrity. Mesoporous silica nanoparticles (MSNs) with and without incorporated stabilizing reagents were used to encapsulate triosephosphate isomerase mRNA of Arabidopsis thaliana. The absorption, elution, and the long-term stability of the RNA were monitored by using quantitative real-time RT-PCR. The results indicate that adsorbed RNA can be eluted from the sorbents using simple buffers and employed directly for downstream molecular diagnostic assays without any further processing. RNA integrity can be maintained for extended time periods under refrigeration temperatures in the presence of covalently immobilized stabilizing compounds. This study provides initial evidence of the potential for application of MSNs in transportation and storage. They may also have utility in sample collection and processing in restrictive environments.


Descriptors :   *ADSORPTION , *RIBONUCLEIC ACIDS , ABSORPTION , ADSORBENTS , ELUTION , NANOPARTICLES , POROUS MATERIALS , SILICATES , STABILIZATION , STORAGE


Subject Categories : Medicine and Medical Research
      Medical Facilities, Equipment and Supplies


Distribution Statement : APPROVED FOR PUBLIC RELEASE