Accession Number : ADA568434


Title :   The Role of Crk Adaptor Proteins in Breast Tumorigenesis and Bone Metastasis


Descriptive Note : Annual summary rept. 1 Sep 2011-31 Aug 2012


Corporate Author : ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING MONTREAL (CANADA)


Personal Author(s) : Bell, Emily


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a568434.pdf


Report Date : Sep 2012


Pagination or Media Count : 30


Abstract : We have established a relationship between Crk protein expression and cell proliferation in basal breast cancer. Stable knockdown of Crk protein expression using shRNA in multiple basal breast cancer cell lines decreased both adhesion-independent growth in vitro and proliferation in vivo. Immunohistochemistry to CrkI/II, CrkL, and the proliferative marker Ki67 revealed a positive correlation between Crk protein levels and the proliferative index of human basal tumors in a tissue microarray. The association of a Crk gene expression signature with basal tumors and poor prognosis further supports a role for Crk-dependent signaling in the growth of basal tumors. These findings support a requirement for Crk protein expression in basal tumor progression, and identify Crk proteins as an attractive subject for further study and therapeutic intervention in basal tumors.


Descriptors :   *ADAPTERS , *BONES , *BREAST CANCER , *METASTASIS , *PROTEINS , CELLS(BIOLOGY) , GENES , GROWTH(PHYSIOLOGY) , HISTOCHEMISTRY , IMMUNOCHEMISTRY , IN VIVO ANALYSIS , MAMMARY GLANDS , NEOPLASMS , STABILITY , THERAPY


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE