Accession Number : ADA568166


Title :   Preclinical Studies of Induced Pluripotent Stem Cell-Derived Astrocyte Transplantation in ALS


Descriptive Note : Annual rept. 30 Sep 2011-29 Sep 2012


Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE


Personal Author(s) : Maragakis, Nicholas J ; Song, Hongjun


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a568166.pdf


Report Date : Oct 2012


Pagination or Media Count : 9


Abstract : We have made substantial progress in obtaining skin biopsies with subsequent fibroblast cultures of a number of sporadic ALS, familial ALS, and control subjects in the last year. We have created numerous iPS cell lines from these patient samples and have characterized their long-term differentiation into astroglia. We now have data from the in vivo transplantation of these astroglial progenitors into rat spinal cords. We have characterized several control and 1 ALS cell line with regard to their survival, differentiation, migration, and phenotypic effects on the host animals. Our data demonstrate that there are differences amongst the cell lines with regard to these properties but we have not appreciated obvious differences with regard to ALS cells when compared with control astroglial cells. Finally, we have demonstrated, in preparation for our therapeutic approaches, that these glial progenitor cells continue to mature into astrocytes following transplantation.


Descriptors :   *ASTROCYTES , *BIOPSY , *CULTURES(BIOLOGY) , *FIBROBLASTS , *STEM CELLS , CELLS(BIOLOGY) , CLINICAL MEDICINE , IN VIVO ANALYSIS , MIGRATION , NERVE CELLS , PATIENTS , PRECURSORS , SPINAL CORD , THERAPY , TRANSPLANTATION


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE