Accession Number:

ADA568162

Title:

FoxP3 as a Missing Link Between Inflammation and Breast Cancer

Descriptive Note:

Revised annual rept. 1 Sep 2010-31 Aug 2011

Corporate Author:

MICHIGAN UNIV ANN ARBOR

Personal Author(s):

• Liu, Yang

Report Date:

2011-09-01

Pagination or Media Count:

36.0

Abstract:

This is the third annual report. The major achievement is establishment of a new mechanism by which Foxp3 activates gene expression. Both H4K16 acetylation and H3K4 tri-methylation are required for gene activation. However, it is still largely unclear how these modifications are orchestrated by transcriptional factors. Here we analyzed the mechanism of the transcriptional activation by FOXP3, an X-linked suppressor of autoimmune diseases and cancers. FOXP3 binds near transcriptional start sites of its target genes. By recruiting MOF and displacing histone H3K4 demethylase PLU-1, FOXP3 increases both H4K16 acetylation and H3K4 tri-methylation at the FOXP3-associated chromatins of multiple FOXP3-activated genes. RNAi-mediated silencing of MOF reduced both gene activation and tumor suppression by FOXP3, while both somatic mutations in clinical cancer samples and targeted mutation of FOXP3 in mouse prostate epithelial disrupted nuclear localization of MOF. Our data demonstrate a pull-push model in which a single transcription factor orchestrates two epigenetic alterations necessary for gene activation and provide a mechanism for somatic inactivation of the FOXP3 protein function in cancer cells.

Descriptors:

• *BREAST CANCER
• *GENES
• *INFLAMMATION
• ACTIVATION
• DISEASES
• EPITHELIUM
• FACTOR ANALYSIS
• FUNCTIONS
• HISTONES
• MUTATIONS
• NEOPLASMS
• PROTEINS
• RECRUITING
• TRANSCRIPTION(GENETICS)

Subject Categories:

• Genetic Engineering and Molecular Biology
• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE