Accession Number : ADA566920


Title :   Fractional Analysis of Escherichia coli O157:H7 by Mass Spectrometry-Based Proteomics


Descriptive Note : Final rept. Oct 2010-Sep 2011


Corporate Author : ARMY EDGEWOOD CHEMICAL BIOLOGICAL CENTER APG MD


Personal Author(s) : Bevilacqua, Vicky L ; Jabbour, Rabih E ; Wade, Mary M ; Deshpande, Samir V ; McCubbin, Patrick E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a566920.pdf


Report Date : Oct 2012


Pagination or Media Count : 22


Abstract : This report is required for the U.S. Army Edgewood Chemical Biological Center In-House Laboratory Independent Research project Membrane Vesicles and Extracellular Proteins in Antibiotic Resistance and Virulence . Certain extracellular proteins of pathogenic bacteria have been shown to function in survival mechanisms such as host immune system modulation (Vranakis, et al., July 27, 2011, J. Proteome Res. DOI 10.1021/pr200422f) and biofilm formation (Ostrowski, A., et al., 2011, J. Bacteriol. 193:4043). To begin to address this possibility, we have analyzed Escherichia coli O157:H7 protein fractions by liquid chromatography-tandem mass spectrometry, followed by biochemical pathway mapping using the Kyoto Encyclopedia of Genes and Genomes. The fimbriae-specific subset included proteins involved in carbohydrate metabolism, which are important in providing energy for fimbriae motion. Moreover, inositol monophosphatase (IMP), which has a role in streptomycin synthesis, and a glucose-specific phosphotransferase system (G-PTS), involved in environmental processing, were also identified. IMP, G-PTS, and a putative stress protein, recently identified in the suspension, are expected to function as part of the bacterial survival mechanisms. This research should provide fundamental knowledge regarding extracellular proteins produced by Gram-negative bacteria. The identification of molecular level components important for survival could prove useful in arenas such as vaccine and antibiotic development.


Descriptors :   *ESCHERICHIA COLI , *GRAM NEGATIVE BACTERIA , *LIQUID CHROMATOGRAPHY , *MASS SPECTROMETRY , *PROTEOMICS , *RICIN , BACTERIA , BIOCHEMISTRY , CARBOHYDRATE METABOLISM , ENCYCLOPEDIAS , MOLECULAR STATES , PROTEINS , SURVIVAL(PERSONNEL) , SUSPENSION DEVICES , VACCINES


Subject Categories : Biochemistry
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE