Accession Number : ADA561076


Title :   Genomic Analysis of Complex Microbial Communities in Wounds


Descriptive Note : Final addendum rept. 2 Jun 2010-1 Jun 2012


Corporate Author : TRANSLATIONAL GENOMICS RESEARCH INST PHOENIX AZ


Personal Author(s) : Price, Lance B


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a561076.pdf


Report Date : Jan 2012


Pagination or Media Count : 48


Abstract : The work that we conducted was aimed at moving wound research beyond the limitations of culture-based microbial analyses to enable comprehensive characterization of wound colonization and to fully evaluate its impact on healing. We successfully developed a novel, culture-independent approach for characterizing wound microbiota; we succeeded in applying statistical tools to begin characterizing the impact of antibiotic therapies and diabetes on wound microbiota; we used our novel strategies to define macro-scale spatial variation in chronic wound microbiota; we established research partnerships with MIHS to conduct prospective cohort studies on burn and chronic wounds; we enrolled a total of 71 patients into a prospective study of burn wound colonization and collected over 700 wound samples from these patients; we developed new protocols to isolate bacterial DNA from burn wounds with minimal exogenous DNA contamination; we developed a novel bioinformatics pipeline for identifying robust real time PCR targets for detection and quantification of clinical pathogens; and developed a comprehensive panel of TaqMan assays (n = 108) for detection of extraintestinal virulence factors in E. coli.


Descriptors :   *BACTERIA , *BURNS(INJURIES) , *CLINICAL MEDICINE , *MICROBIOLOGY , *WOUNDS AND INJURIES , COLONIES(BIOLOGY) , COMMUNITIES , CONTAMINATION , DEOXYRIBONUCLEIC ACIDS , DIABETES , ESCHERICHIA COLI , GENOME , PATHOGENIC MICROORGANISMS , PATIENTS


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE