Accession Number : ADA560759


Title :   Impact of Medium on the Development and Physiology of Pseudomonas fluorescens Biofilms on Polyurethane Paint


Descriptive Note : Final rept. 14 Jan 2009-14 January 2012


Corporate Author : AIR FORCE RESEARCH LAB WRIGHT-PATTERSON AFB OH MATERIALS AND MANUFACTURING DIRECTORATE


Personal Author(s) : Crookes-Goodson, Wendy J ; Mirau, Peter A ; Bojanowski, Caitlin L ; Kay, Michelle L ; Lloyd, Pamela F ; Blankemeier, Andrew ; Fraser, Hamish L ; Barlow, Daniel E ; Pehrsson, Pehr E ; Russell, Jr, John N


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a560759.pdf


Report Date : Feb 2012


Pagination or Media Count : 30


Abstract : Since the 1950s, the Air Force has battled microbial contamination of its fuel systems. Within these systems, microbes are commonly found as biofilms adhered to tank coatings. Thus strategies for mitigating microbial contamination of fuel systems include deterring biofilm growth on coatings. This study investigated how Pseudomonas fluorescens biofilms grow on polyurethane coatings, and contribute to the degradation of those coatings. Specifically, we characterized how medium conditions and substrate composition contribute to growth of P. fluorescens on an antistatic polyurethane coating. We found that P. fluorescens biofilms grew in systems where the only carbon source was the coupon, suggesting that P. fluorescens can use polyurethane as a nutrient source. In addition, we examined the impact of substrate on biofilm growth by quantitating biofilm growth on pigmented and non-pigmented paint. There was no difference in the ability of the biofilm to grow on the pigmented vs. non-pigmented paint, suggesting that the biofilm was utilizing the polyurethane and not the pigment as a carbon source.


Descriptors :   *AVIATION FUELS , *BACTERIA , *BIODEGRADATION , *COATINGS , *PSEUDOMONAS FLUORESCENS , AIR FORCE , CARBON , CONCENTRATION(COMPOSITION) , CONTAMINATION , DEGRADATION , GROWTH(GENERAL) , IMPACT , MICROORGANISMS , NUTRIENTS , ORGANIC MATERIALS , PAINTS , PHYSIOLOGY , PIGMENTS , POLYURETHANE RESINS , SKILLS , SOURCES , STRATEGY , SUBSTRATES , TANKS(CONTAINERS)


Subject Categories : Microbiology
      Coatings, Colorants and Finishes


Distribution Statement : APPROVED FOR PUBLIC RELEASE