Accession Number : ADA554588


Title :   Functional Genomics for Epithelial-Mesenchymal Transition in Breast Cancer


Descriptive Note : Annual rept. 3 Sep 2010-2 Sep 2011


Corporate Author : MELBOURNE UNIV PARKVILLE (AUSTRALIA)


Personal Author(s) : Thompson, Erik


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a554588.pdf


Report Date : Oct 2011


Pagination or Media Count : 40


Abstract : To assess whether modulation of the PMC42-LA cell line by a library of shRNAmir constructs can cause tumorigenicity, the impact of a smaller boutique library is first being assessed, as proposed. This boutique library of 4,462 shRNA constructs targeting 1,860 markers and mediators of EMT, metastasis, migration, and BCSC has been transduced into the PMC42-LA cell line as a total pool and as ten smaller sub-pools. These were expanded in preparation for in vivo testing. The smaller sub-pools have greater representation of individual shRNAmir constructs. For any sub-pool containing shRNAmir constructs that enables tumorigenicity, this greater representation translates to a markedly larger inoculum of tumorigenic cells, which may be important in the establishment of a tumor. The presence of a small but significant proportion of colonies exhibiting markedly mesenchymal features has been observed in the transduced pools, validating the use of this boutique library pool to directly assess whether PMC42-LA tumors can be grown using pools containing a proportion of more mesenchymal cells as an inoculum prior to assessing the whole genome shRNAmir library. Various methodologies for the in vitro selection of transduced cells with mesenchymal features from these pools, supplementary to the initial aims, are under development.


Descriptors :   *BREAST CANCER , *CELLS(BIOLOGY) , *EPITHELIUM , *METASTASIS , *RIBONUCLEIC ACIDS , COLONIES(BIOLOGY) , CONNECTIVE TISSUE , EMBRYOS , GENOME , IN VITRO ANALYSIS , IN VIVO ANALYSIS , LIBRARIES , MIGRATION , NEOPLASMS , TEST AND EVALUATION


Subject Categories : Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE