Accession Number : ADA553045


Title :   Cloning and Expressing Recombinant Protective Antigen Domains of B. anthracis


Descriptive Note : Rept. for Oct 2010-Jul 2011


Corporate Author : ARMY RESEARCH LAB ADELPHI MD SENSORS AND ELECTRON DEVICES DIRECTORATE


Personal Author(s) : Sarkes, Deborah A ; Kogot, Joshua M ; Val-Addo, Irene ; Stratis-Cullum, Dimitra N ; Pellegrino, Paul M


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a553045.pdf


Report Date : Sep 2011


Pagination or Media Count : 26


Abstract : Protective antigen (PA) of Bacillus anthracis is a four-domain protein that is required for transport of edema factor (EF) and lethal factor (LF) into host cells during anthrax infection. Each of the four domains of PA plays a role in infection: host cell receptor binding, domain 4, PA heptamerization and host membrane insertion, domains 2 and 3, and EF and LF recognition and intracellular transport, domain 1. This report details our efforts to produce single- and multi-domain combinations of the PA protein. All multi-domains (1-2, 2-3, 3-4) and three of the four single domains (1, 2, 4) have been cloned, expressed, and purified and will be used for future efforts in domain mapping of PA-binding antibodies and peptide reagents.


Descriptors :   *BACILLUS ANTHRACIS , *PROTEINS , ANTIGENS


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE