Accession Number : ADA552238


Title :   Antigen-Specific lgA B Memory Cell Responses to Shigella Antigens Elicited in Volunteers Immunized with Live Attenuated Shigella flexneri 2a Oral Vaccine Candidates


Descriptive Note : Journal article


Corporate Author : NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD


Personal Author(s) : Simon, J K ; Maciel Jr, M ; Weld, E D ; Wahid, R ; Pasetti, M F ; Picking, W L ; Kotloff, K L ; Levine, M M ; Sztein, M B


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a552238.pdf


Report Date : Jan 2011


Pagination or Media Count : 9


Abstract : We studied the induction of antigen-specific lgA memory B cells (BM) in volunteers who received live attenuated Shigella flexneri 2a vaccines. Subjects ingested a single oral dose of 107 , 108 or 109 CFU of S. flexneri 2a with deletions in guaBA (CVD 1204) or in guaBA, set and sen (CVD 1208). Antigen-specific serum and stool antibody responses to LPS and lpa B were measured on days 0, 7, 14, 28 and 42. lgA BM cells specific to LPS, lpa Band totallgA were assessed on days 0 and 28. We show the induction of significant LPS-specific lgA BM cells in anti-LPS lgA seroresponders. Positive correlations were found between anti-LPS lgA BM cells and anti-LPS lgA in serum and stool; lgA BM cell responses to lpaB were also observed. These BM cell responses are likely play an important role in modulating the magnitude and longevity of the humoral response.


Descriptors :   *ANTIGENS , *B LYMPHOCYTES , *RESPONSE(BIOLOGY) , *SHIGELLA FLEXNERI , *VACCINES , BLOOD SERUM , CHILDREN , DOSAGE , INDUCTION SYSTEMS , MEMORY DEVICES , ORAL INTAKE


Subject Categories : Medicine and Medical Research
      Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE