Accession Number : ADA552048


Title :   Articular Cartilage Repair Through Muscle Cell-Based Tissue Engineering


Descriptive Note : Annual rept. 1 Mar 2010-28 Feb 2011


Corporate Author : CHILDREN'S HOSPITAL OF PITTSBURGH PA


Personal Author(s) : Huard, Johnny


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a552048.pdf


Report Date : Mar 2011


Pagination or Media Count : 86


Abstract : Using the modified preplate technique, we have isolated a population of early myogenic progenitor cells from postnatal skeletal muscle that display stem cell characteristics. We have shown that these muscle-derived stem cells (MDSCs) can differentiate toward myogenic, osteogenic, chondrogenic, neurogenic, and hematopoietic lineages. Specifically, MDSCs cultured in chondrogenic medium can undergo chondrogenic differentiation in vitro, and MDSCs delivered to osteochondral defects display good cell survival and can differentiate into chondrocytes that improve the healing of articular cartilage. We also have observed that bone morphogenetic protein 4 (BMP4) promotes chondrogenic differentiation of MDSCs in vitro and in vivo and that this cytokine can trigger chondrogenic differentiation of other populations of muscle-derived cells, including myoblasts (late myogenic progenitor cells) and fibroblasts. The proposed project will investigate the use of these populations of muscle-derived cells as novel cell sources for articular cartilage repair in osteochondral defects created in nude rats. We will investigate the in vitro chondrogenic potential of various populations of mouse muscle-derived cells (fibroblastic cells and early and late myogenic progenitor cells) expressing BMP4 and determine the regenerative capacity of these cells after implantation in rat articular cartilage defects. We then will explore the relative contributions of these muscle-derived cells long-term proliferation, survival, and self-renewal to their regenerative capacity after transplantation into the cartilage defects (Technical Objective #1). Next, we will determine the contributions of angiogenesis and scar tissue formation to the regenerative capacity of the muscle-derived cells and to the overall quality of the cartilage generated within the treated osteochondral defects (Technical Objective #2).


Descriptors :   *CARTILAGE , *MUSCLES , *MUSCULOSKELETAL SYSTEM , ANGIOGENESIS , BONES , CELLS(BIOLOGY) , DEFECTS(MATERIALS) , GENETIC ENGINEERING , HEALING , IMPLANTATION , REPAIR , SCARS , STEM CELLS , SURVIVABILITY , TISSUES(BIOLOGY) , TRANSPLANTATION


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE