Accession Number : ADA552031


Title :   PFGE, Lior Serotype, and Antimicrobial Resistance Patterns Among Campylobacter jejuni Isolated from Travelers and US Military Personnel with Acute Diarrhea in Thailand, 1998-2003


Descriptive Note : Journal article


Corporate Author : NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD


Personal Author(s) : Serichantalergs, Oralak ; Pootong, Piyarat ; Dalsgaard, Anders ; Bodhidatta, Ladaporn ; Guerry, Patricia ; Tribble, David R ; Anuras, Sinn ; Mason, Carl J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a552031.pdf


Report Date : Jan 2010


Pagination or Media Count : 13


Abstract : Background: Campylobacter jejuni is a major cause of gastroenteritis worldwide. In Thailand, several strains of C. jejuni have been isolated and identified as major diarrheal pathogens among adult travelers. To study the epidemiology of C. jejuni in adult travelers and U.S. military personnel with acute diarrhea in Thailand from 1998-2003, strains of C. jejuni were isolated and phenotypically identified, serotyped, tested for antimicrobial susceptibility, and characterized using pulsed-field gel electrophoresis (PFGE). Results: A total of 312 C. jejuni isolates were obtained from travelers (n = 46) and U.S. military personnel (n = 266) in Thailand who were experiencing acute diarrhea. Nalidixic acid and ciprofloxacin resistance was observed in 94.9% and 93.0% of the isolates, respectively. From 2001-2003, resistance to tetracycline (81.9%), trimethoprimsulfamethoxazole (57.9%), ampicillin (28.9%), kanamycin (5.9%), sulfisoxazole (3.9%), neomycin (2.0%), and streptomycin (0.7%) was observed. Combined PFGE analysis showed considerable genetic diversity among the C. jejuni isolates; however, four PFGE clusters included isolates from the major Lior serotypes (HL: 36, HL: 11 , HL: 5 and HL: 28). The PFGE analysis linked individual C. jejuni clones that were obtained at U.S. military exercises with specific antimicrobial resistance patterns. Conclusions: In summary, most human C. jejuni isolates from Thailand were multi-resistant to quinolones and tetracycline. PFGE detected spatial and temporal C. jejuni clonality responsible for the common sources of Campylobacter gastroenteritis.


Descriptors :   *ANTIBIOTICS , *BACTERIA , *DIARRHEA , MILITARY PERSONNEL , PATHOGENIC MICROORGANISMS , REPRINTS , RESISTANCE(BIOLOGY) , THAILAND


Subject Categories : Microbiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE