Accession Number : ADA551717


Title :   Comparative Analysis of Angiogenic Gene Expression in Normal and Impaired Wound Healing in Diabetic Mice: Effects of Extracorporeal Shock Wave Therapy


Descriptive Note : Journal article


Corporate Author : NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD


Personal Author(s) : Zins, Stephen R ; Amare, Mihret E ; Tadaki, Douglas K ; Elster, Eric A ; Davis, Thomas A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a551717.pdf


Report Date : Jan 2010


Pagination or Media Count : 14


Abstract : Impaired wound healing is a persistent clinical problem which has been treated with mixed results. Studies aimed at elucidating the mechanism of impaired wound healing have focused on small cohorts of genes which leave an incomplete picture of the wound healing process. We aimed to investigate impaired wound healing via a comprehensive panel of angiogenic/inflammation-related genes and wound closure kinetics with and without the application of extracorporeal shock wave therapy (ESWT), which has been demonstrated to improve wound healing. Full-thickness skin from the dorsal surface of normal (BALB/c) and impaired (db+/db+) mice was excised, and wound margin tissue was harvested 2, 7, and 10 days post injury. A separate but identical wound model was established over 40 days in order to measure wound closure kinetics. Over time, the normal non-ESWT treated wounds exhibited varying patterns of elevated expression of 25-30 genes whereas wounds with impaired healing displayed prolonged elevated expression of only a few genes (CXCL2, CXCL5 CSF3, MMP9, TGF-alpha). In response to ESWT, gene expression was augmented in both types of wounds, especially in the expression of PECAM-1; however, ESWT had no effect on wound closure in either model. In addition, multiple doses of ESWT exacerbated the delayed wound healing, and actually caused the wounds to initially increase in size. These data provide a more complete picture of impaired wound healing, and a way to evaluate various promising treatments.


Descriptors :   *ANGIOGENESIS , *DIABETES , *GENE EXPRESSION , *GENES , *WOUNDS AND INJURIES , AUGMENTATION , CLINICAL MEDICINE , DISPLAY SYSTEMS , DOSAGE , HEALING , MICE , RESPONSE(BIOLOGY) , SHOCK THERAPY , SHOCK WAVES , TISSUES(BIOLOGY)


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE