Accession Number : ADA549276


Title :   Early Detection of Breast Cancer Using Posttranslationally Modified Biomarkers


Descriptive Note : Annual summary 1 Mar 2010-28 Feb 2011


Corporate Author : BATTELLE MEMORIAL INST RICHLAND WA PACIFIC NORTHWEST LABS


Personal Author(s) : Jin, Hongjun


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a549276.pdf


Report Date : Mar 2011


Pagination or Media Count : 20


Abstract : In this project, we focus on oxidation and glycosylation PTMs on proteins known to be secreted by the breast as candidate biomarkers for the early detection of breast cancer. ELISA microarray technology is employed to evaluate assays that have potential be used as breast cancer biomarkers. During the first year of this project we have validated a panel of ELISA capture antibodies for our PTM-ELISA microarray. And we tested 8 proposed PTM detection antibodies with clinical breast cancer plasma samples. Nitrotyrosine, bromotyrosine, chlorotyrosine, TF antigens, and AEG do not give significant difference in our test with clinical breast cancer samples. GSH, GloboH and 4-HNE were found to be altered with breast cancer plasmas samples. The sensitivity and the specificity of each assay and the combined assays were evaluated using the ROC analysis. In the best multivariate analysis, the AUC values ranged from 78% to 89% for 3 different PTMs (GSH, GloboH and 4-HNE). Overall, our data suggest that the PTM ELISA microarray platform is a promising tool for discovery and evaluation of biomarkers that have potential for the early detection of breast cancer.


Descriptors :   *BREAST CANCER , ANTIBODIES , CLINICAL MEDICINE , DETECTION , GLYCOSIDES , MAMMARY GLANDS , MARKERS , MULTIVARIATE ANALYSIS , PROTEINS , SAMPLING , SENSITIVITY , TEST AND EVALUATION


Subject Categories : Anatomy and Physiology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE