Accession Number : ADA548699


Title :   Pathological Fingerprints, Systems Biology and Biomarkers of Blast Brain Injury


Descriptive Note : Annual rept. 15 May 2009-14 May 2010


Corporate Author : BANYAN BIOMARKERS INC ALACHUA FL


Personal Author(s) : Svetlov, Stanislav


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a548699.pdf


Report Date : Jun 2010


Pagination or Media Count : 50


Abstract : We compared the effects of primary blast overpressure exposure of controlled duration, peak pressure, and transmitted impulse with brain injury by a severe blast load accompanied with strong head acceleration. The off-axis rat positioning avoided strong head acceleration due to the blast generator venting gas impulse, and reproduced primary blast with different mechanisms. sICAM, L-selectin, and E-selectin accumulated substantially in rat blood 24 hours after the blast and persisted for 14 days post-blast. Serum matrix metalloproteinases (MMP) exhibited a similar profile. Nerve growth factor beta-NGF in blood was significantly elevated within the first week post-blast and showed the most pronounced changes when the total animal body was subjected to blast waves. Resistin accumulated in rat blood 7 days after blast followed by a gradual decline. Neuropeptide Orexin A content showed a drastic rise at 24 hours after a blast that targeted the total body, followed by a gradual decline. In contrast, a blast wave targeting only the animal's head caused a gradual raise of Orexin through 30 days post-exposure. For all biomarkers, the detected levels rose in all the setups studied. Nevertheless, the most significant and persistent serum changes were observed when the total animal body was subjected to a blast wave compared to setups in which only the animal's head was targeted. Proteomic profiling of novel biomarkers has been performed and the results are being analyzed.


Descriptors :   *BLAST , *MARKERS , *MOLECULAR BIOLOGY , *PATHOLOGY , *RATS , *SHOCK WAVES , *TRAUMATIC BRAIN INJURIES , BIOASSAY , BLOOD BRAIN BARRIER , BLOOD SERUM , CEREBROSPINAL FLUID , ENDOTHELIUM , ENZYMES , HEAD(ANATOMY) , INFLAMMATION , MACROPHAGES , NERVE CELLS , OVERPRESSURE , PROTEINS , RESPONSE(BIOLOGY) , SHOCK TUBES


Subject Categories : Biochemistry
      Genetic Engineering and Molecular Biology
      Medicine and Medical Research
      Weapons Effects(biological)


Distribution Statement : APPROVED FOR PUBLIC RELEASE