Accession Number : ADA542906


Title :   Distinct Circulating Recombinant HIV-1 Strains Among Injecting Drug Users and Sex Workers in Afghanistan


Descriptive Note : Journal article


Corporate Author : WALTER REED ARMY INST OF RESEARCH ROCKVILLE MD US MILITARY HIV RESEARCH PROGRAM/DIVISION OF RETROVIROLOGY


Personal Author(s) : Sanders-Buell, Eric ; Bose, Meera ; Nasir, Abdul ; Todd, Catherine S ; Stanekzai, M R ; Tovanabutra, Sodsai ; Scott, Paul T ; Strathdee, Steffanie A ; Tjaden, Jeffrey ; Michael, Nelson L ; McCutchan, Francine E


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a542906.pdf


Report Date : May 2010


Pagination or Media Count : 5


Abstract : Little information is available regarding a circulating HIV genotype among high-risk groups in Afghanistan; we describe HIV genotypes among injecting drug users (IDUs) and sex workers (SWs) in four Afghan cities. Participants completed behavioral questionnaires and HIV testing. Western blot-confirmed specimens had peripheral mononuclear blood cells isolated for genotyping. Analysis of recombinants was done by bootscanning and manual sequence alignment. The single SW sample harbored a CRF01_AE strain. Of 10 IDUs available for analysis, all were CRF35_AD and from Hirat. Analyzed subregions (gag p17 and env C1-C5) revealed close homology between the Hirat specimens. Three distinct subclusters comprising two or three strains were identified whereas two other strains were generally equidistant from previously identified Kabul strains. Results suggest that the nascent HIV epidemic among IDUs in Hirat is largely, if not entirely, subtype CRF35_AD, and the close homology suggests recent infection; harm reduction should be supported to avert further transmission.


Descriptors :   *DRUG USERS , *HUMAN IMMUNODEFICIENCY VIRUSES , *INFECTIOUS DISEASES , MANUAL OPERATION , PERSONNEL , AFGHANISTAN , QUESTIONNAIRES , SEX , SAMPLING , RISK , REPRINTS , BEHAVIOR


Subject Categories : Sociology and Law
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE