Accession Number : ADA539721


Title :   Characterization and In Vitro Toxicity of Copper Nanoparticles (Cu-NPs) in Murine Neuroblastoma (N2A) Cells


Descriptive Note : Master's thesis


Corporate Author : AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH DEPT OF SYSTEMS AND ENGINEERING MANAGEMENT


Personal Author(s) : Brownheim, Sitao V


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a539721.pdf


Report Date : Mar 2011


Pagination or Media Count : 112


Abstract : Nanomaterials, classified as materials within the 1 - 100 nanometer range, have seemingly endless applications. It is very important to the Air Force to increase the understanding of Cu-NPs and the possible health impacts from nanomaterial exposure prior to wide-spread industry and military use. Therefore, the purpose of this research is to thoroughly characterize the properties of three different copper samples upon suspension into aqueous solutions over time: Cu-NPs (Cu 25 nm), CuO-NPs (CuO 40 nm), and Cu-MSP (Cu 500nm). Additionally, this research seeks to observe in vitro effects upon murine neuroblastoma cells (N2A) by these three types of particles as well as by CuCl2. Microscopy techniques indicate observable changes in oxidation for Cu-NP 25 nm and CuO-NP 40 nm after suspension in water over long incubation. In general, agglomerations of the particles increase when dosed with the endocytosis inhibitor Dynasore. In terms of cellular viability, CuCl2 is the least toxic at all concentrations. Cu-NP 25 nm is less toxic at low concentrations ( 25 micrograms/mL) than Cu-MSP 500 nm and CuO-NP 40 nm. At concentrations above 25 micrograms/mL, Cu-NP 25 nm becomes comparable in toxicity to CuO-NP 40 nm and more toxic than Cu-MSP 500 nm.


Descriptors :   *NANOPARTICLES , *TOXICITY , *PHYSICOCHEMICAL PROPERTIES , *COPPER , MICE , NEUROBLASTOMA , ENVIRONMENTAL IMPACT , MICROSCOPY , IN VITRO ANALYSIS , THESES , OXIDATION


Subject Categories : Biochemistry
      Toxicology
      Environmental Health and Safety


Distribution Statement : APPROVED FOR PUBLIC RELEASE