Accession Number : ADA539011


Title :   Evidence of Genetic Differentiation for Hawaii Insular False Killer Whales (Pseudorca crassidens)


Corporate Author : CASCADIA RESEARCH COLLECTIVE OLYMPIA WA


Personal Author(s) : Chivers, Susan J ; Baird, Robin W ; Martien, Karen M ; Taylor, Barbara L ; Archer, Eric ; Gorgone, Antoinette M ; Hancock, Brittany L ; Hedrick, Nicole M ; Matilla, David ; McSweeney, Daniel J


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a539011.pdf


Report Date : May 2010


Pagination or Media Count : 51


Abstract : Chivers et al. (2007) found Hawai'i insular false killer whales to be distinct from other strata within the Indo-Pacific Ocean using mitochondrial DNA (mtDNA) control region sequence data. Here, we add new samples and eight nuclear DNA (nDNA) microsatellite markers to that study. After extensive quality checking, some haplotypes and duplicate individuals were removed from the 2007 mtDNA data set. A strong phylogeographic signal consistent with local haplotype evolution was evident for Hawai'i insular false killer whales with all but one individual having one of 2 closely related haplotypes found only in this population. The mtDNA characteristics of the Hawai'i insular false killer whales (n = 81) differed significantly (all p-values for Fisher exact and phiST 0.0001) from both broad-scale strata (Central North Pacific (n = 13) and Eastern North Pacific (n = 39)), and all fine-scale strata (Hawai'i pelagic (n = 9), Mexico (n = 19), Panama (n = 15) and American Samoa (n = 6)). The magnitude of mtDNA differentiation (all phiST 0.68) was consistent with less than one migrant per generation. The nDNA marker results were highly significant with all Fisher exact p-values less than or equal to 0.001 for comparisons of the Hawai'i insular stratum (n = 69) to the broad-scale strata (Central North Pacific (n = 13) and Eastern North Pacific (n = 36)), and fine-scale strata (Hawai'i pelagic (n = 9), Mexico (n = 19), Panama (n = 12) and American Samoa (n = 6)). The magnitude of differentiation was much less for nDNA (0.01 FST 0.08, 0.01 Jost's D 0.06) than for mtDNA, indicating the potential for some male-mediated gene flow although the possibility that FST is low because of the high mutation rate of microsatellites or the influence of selection operating to counter gene flow cannot be excluded.


Descriptors :   *WHALES , *DEOXYRIBONUCLEIC ACIDS , MITOCHONDRIA , GENETICS , EVOLUTION(GENERAL) , MUTATIONS , HAWAII


Subject Categories : Genetic Engineering and Molecular Biology
      Biology


Distribution Statement : APPROVED FOR PUBLIC RELEASE