Accession Number : ADA530303


Title :   Rapid, Potentially Automatable, Method Extract Biomarkers for HPLC/ESI/MS/MS to Detect and Identify BW Agents


Descriptive Note : Conference paper


Corporate Author : OAK RIDGE NATIONAL LAB TN


Personal Author(s) : White, D C ; Burkhalter, R S ; Smith, C ; Macnaughton, S ; Whitaker, K W


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a530303.pdf


Report Date : Nov 1997


Pagination or Media Count : 12


Abstract : Our program proposes to concentrate on the rapid recovery of signature biomarkers based on automated high-pressure, high-temperature solvent extraction (ASE) and/or supercritical fluid extraction (SFE) to produce lipids, nucleic acids and proteins sequentially concentrated and purified in minutes with yields especially from microeukaryotes, Gram-positive bacteria and spores. Lipids are extracted in higher proportions greater than classical one-phase, room temperature solvent extraction without major changes in lipid composition. High performance liquid chromatography (HPLC) with or without derivatization, electrospray ionization (ESI) and highly specific detection by mass spectrometry (MS) particularly with ((MS)exp n) provides the detection, identification and because the signature lipid biomarkers are both phenotypic as well as genotypic biomarkers, insights into potential infectivity of BW agents. Feasibility has been demonstrated with detection, identification, and determination of infectious potential of Cryptosporidium parvum at the sensitivity of a single oocyst (which is unculturable in vitro) and accurate identification and prediction, pathogenicity, and drug-resistance of Mycobacteria spp.


Descriptors :   *BIOLOGICAL AGENT DETECTORS , LIPIDS , PROTEINS , SPORES , LIQUID CHROMATOGRAPHY , IONIZATION , GRAM POSITIVE BACTERIA , BIOLOGICAL WARFARE AGENTS , OOCYSTS , NUCLEIC ACIDS , MASS SPECTROMETRY , MYCOBACTERIUM , SYMPOSIA , ANTHRAX


Subject Categories : Biochemistry
      Chemical, Biological and Radiological Warfare


Distribution Statement : APPROVED FOR PUBLIC RELEASE