Accession Number : ADA524504


Title :   The Role of Central Metabolism in Prostate Cancer Progression


Descriptive Note : Annual rept. 15 Sep 2008-15 Sep 2009


Corporate Author : PITTSBURGH UNIV PA


Personal Author(s) : Conrads, Thomas P


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a524504.pdf


Report Date : Oct 2009


Pagination or Media Count : 12


Abstract : We hypothesize that by enriching the diet with omega-3 PUFAs PCa tumor progression will be significantly reduced. Patients with localized PCa will be enrolled in a randomized, doubleblinded phase-I clinical trial in which they will be given omega-3 fish oil or control oleic acid supplements 5 weeks prior to prostatectomy. We will evaluate differential protein and phosphopeptide expression in PCa cells, obtained from laser capture microdissected tissue, by mass spectrometry. As primary endpoints, we will compare proliferation and apoptosis in tumors from each group using immunohistochemistry and DNA nick end labeling. As secondary endpoints, we will compare serum prostate specific antigen and hormone levels. We will also measure levels of dietary PUFAs in red blood cell membranes as well as FASN and PUFA metabolic products in prostate tissue using high performance liquid chromatography, mass spectrometry, and gas chromatography.


Descriptors :   *METABOLISM , *PROSTATE CANCER , PROTEINS , NEOPLASMS , DIET , ANTIGENS , GAS CHROMATOGRAPHY , MASS SPECTROMETRY , OLEIC ACID , MEMBRANES(BIOLOGY) , HORMONES , LIQUID CHROMATOGRAPHY


Subject Categories : Biochemistry
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE