Accession Number : ADA523449


Title :   Monitoring the Healing of Combat Wounds Using Raman Spectroscopic Mapping


Descriptive Note : Journal article


Corporate Author : NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD


Personal Author(s) : Crane, Nicole J ; Brown, Trevor S ; Evans, Korboi N ; Hawksworth, Jason S ; Hussey, Sean ; Tadaki, Doug K ; Elster, Eric A


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a523449.pdf


Report Date : Jan 2010


Pagination or Media Count : 10


Abstract : Soldiers wounded in modern warfare present with extensive and complicated acute wounds, confounded by an overwhelming inflammatory response. The pathophysiology of acute wounds is unknown and timing of wound closure remains subjective. Collagen gene expression profiles are presented for 24 patients. Impaired healing wounds show a twofold decrease in the up-regulation of COL1A1 and COL3A1 genes in the beginning of the wound healing process compared with normal healing wounds. By the final debridement, however, collagen gene expression profiles for normal and impaired healing wounds are similar for COL1A1 and COL3A1. In addition, Raman spectroscopic maps are collected of biopsy tissue sections, from the first and last debridements of 10 wounds collected from nine patients. Tissue components obtained for the debridement biopsies are compared to elucidate whether or not a wound heals normally. Raman spectroscopy showed a loss of collagen in five patients, indicated by a negative percent difference in the 1,665/1,445cm(exp.-1) band area ratios. Four healed patients showed an increased or unchanged collagen content. Here, we demonstrate the potential of Raman spectroscopic analysis of wound biopsies for classification of wounds as normal or impaired healing. Raman spectroscopy has the potential to noninvasively monitor collagen deposition in the wound bed, during surgical wound debridements, to help determine the optimal time for wound closure.


Descriptors :   *WOUNDS AND INJURIES , *RAMAN SPECTROSCOPY , GENE EXPRESSION , HEALING , BIOPSY , COLLAGEN , CLASSIFICATION , MILITARY PERSONNEL , PATHOPHYSIOLOGY


Subject Categories : Medicine and Medical Research
      Weapons Effects(biological)
      Anatomy and Physiology


Distribution Statement : APPROVED FOR PUBLIC RELEASE