Accession Number : ADA519474


Title :   Test for Chemical Induction of Chromosome Aberration in Cultured Chinese Hamster Ovary (CHO) Cells With and Without Metabolic Activation. Test Article: N,N,N',N'-tetramethyl Ethanediamine (TMEDA)


Descriptive Note : Final


Corporate Author : SITEK RESEARCH LABS ROCKVILLE MD


Personal Author(s) : Song, Jian


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/a519474.pdf


Report Date : 13 Jun 2008


Pagination or Media Count : 73


Abstract : N,N,N',N'-tetramethyl ethanediamine (TMEDA, 99.86% pure) was tested for its potentials to induce chromosome aberrations in cultured Chinese Hamster Ovary (CHO) cells with and without metabolic activation according to OECD TG 473 in compliance with Good Laboratory Practice. TMEDA was tested at concentrations of 500, 1000, 2500 and 5000 micrograms/mL in both with and without activation. It produced a positive response in this system with or without metabolic activation, but only at the highest concentration 5,000 micrograms/mL However, according to the OECD guidelines TG 473, the compound is considered to be negative in the CHO chromosomal aberration assay, since the compound is not clastogenic at 0.01M (1,140 micrograms/mL). A confirmatory chromosome aberration assay was performed without activation also showed negative at concentrations up to 3,000 micrograms/mL but positive at the highest concentration.


Descriptors :   *HAMSTERS , *OVARIES , *CHROMOSOMES , *RESPONSE(BIOLOGY) , INDUCTION SYSTEMS , CULTURES(BIOLOGY) , MUTATIONS , METABOLISM , CHEMICALS , LABORATORIES


Subject Categories : Biology
      Medicine and Medical Research


Distribution Statement : APPROVED FOR PUBLIC RELEASE